Mesenchymal stromal cells suspended in a platelet lysate gel improve wound healing in mice

Luciana da Rosa Zinn Sostizzo; Raul Marques Rodrigues; Tuane Nerissa Alves Garcez; Victor de Mello Palma; Annelise Martins Pezzi da Silva; Vanessa de Souza Valim; Ana Helena da Rosa Paz; Anelise Bergmann Araujo; Alessandra Aparecida Paz; Lucia Silla

Autores

Luciana da Rosa Zinn Sostizzo Hospital de Clínicas de Porto Alegre – Porto Alegre (RS), Brasil. https://orcid.org/0000-0002-1721-7906
Raul Rodrigues Universidade Federal do Rio Grande do Sul, Faculdade de Medicina, Programa de Pós-Graduação em Ciências Médicas – Porto Alegre (RS), Brasil. https://orcid.org/0000-0003-4436-2153
Tuane Nerissa Alves Garcez Hospital de Clínicas de Porto Alegre – Porto Alegre (RS), Brasil. https://orcid.org/0000-0001-7226-5364
Victor de Mello Palma Universidade Federal do Rio Grande do Sul, Faculdade de Odontologia – Porto Alegre (RS), Brasil. https://orcid.org/0000-0002-8853-0270
Annelise Martins Pezzi da Silva Hospital de Clínicas de Porto Alegre – Porto Alegre (RS), Brasil. https://orcid.org/0000-0002-4929-7525
Vanessa de Souza Valim Hospital de Clínicas de Porto Alegre – Porto Alegre (RS), Brasil. https://orcid.org/0000-0003-1738-0807
Ana Helena da Rosa Paz Hospital de Clínicas de Porto Alegre – Porto Alegre (RS), Brasil. https://orcid.org/0000-0003-3668-7054
Anelise Bergmann Araujo Hospital de Clínicas de Porto Alegre – Porto Alegre (RS), Brasil. https://orcid.org/0000-0001-5277-1964
Alessandra Aparecida Paz Hospital de Clínicas de Porto Alegre – Porto Alegre (RS), Brasil. https://orcid.org/0000-0001-6429-3998
Lucia Silla Hospital de Clínicas de Porto Alegre – Porto Alegre (RS), Brasil. https://orcid.org/0000-0002-3892-6525

Resumo

Objetivo: Avaliar a segurança e a eficácia do tratamento tópico de feridas com células estromais mesenquimais derivadas da medula óssea cultivadas e suspensas em lisado plaquetário. Método: Estudo prospectivo intervencionista e controlado com 42 camundongos C57BL/6 com ferida excisional induzida. Eles foram divididos em quatro grupos de tratamento: hidrogel (tratamento padrão de feridas), gel salino (controle), gel de lisado de plaquetas e gel de lisado de plaquetas contendo as células estromais mesenquimais. As análises foram feitas no dia 7 (n=5) e no dia 13 (n=7) para imuno-histoquímica, e no dia 21 (n=30) para segurança. O acompanhamento ocorreu por meio de avaliação clínica (eventos adversos, peso, dor, comportamento, redução do tamanho da ferida) e histológica (cicatrização, vascularização, colagenização). Resultados: Em relação à segurança, não foram identificados eventos adversos ou alterações fisiológicas relacionados ao tratamento. O grupo tratado com células apresentou melhores resultados na cicatrização através das avaliações macro e microscópicas, seguido pelo grupo gel de lisado plaquetário. Conclusão: A aplicação tópica de células estromais mesenquimais foi segura e mostrou resultados favoráveis.

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