Mesenchymal stromal cells suspended in a platelet lysate gel improve wound healing in mice

Luciana da Rosa Zinn Sostizzo; Raul Marques Rodrigues; Tuane Nerissa Alves Garcez; Victor de Mello Palma; Annelise Martins Pezzi da Silva; Vanessa de Souza Valim; Ana Helena da Rosa Paz; Anelise Bergmann Araujo; Alessandra Aparecida Paz; Lucia Silla

Autores/as

Luciana da Rosa Zinn Sostizzo Hospital de Clínicas de Porto Alegre – Porto Alegre (RS), Brasil. https://orcid.org/0000-0002-1721-7906
Raul Rodrigues Universidade Federal do Rio Grande do Sul, Facultad de Medicina, Programa de Posgrado en Ciencias Médicas – Porto Alegre (RS), Brasil. https://orcid.org/0000-0003-4436-2153
Tuane Nerissa Alves Garcez Hospital de Clínicas de Porto Alegre – Porto Alegre (RS), Brasil. https://orcid.org/0000-0001-7226-5364
Victor de Mello Palma Universidade Federal do Rio Grande do Sul, Facultad de Odontología – Porto Alegre (RS), Brasil. https://orcid.org/0000-0002-8853-0270
Annelise Martins Pezzi da Silva Hospital de Clínicas de Porto Alegre – Porto Alegre (RS), Brasil. https://orcid.org/0000-0002-4929-7525
Vanessa de Souza Valim Hospital de Clínicas de Porto Alegre – Porto Alegre (RS), Brasil. https://orcid.org/0000-0003-1738-0807
Ana Helena da Rosa Paz Hospital de Clínicas de Porto Alegre – Porto Alegre (RS), Brasil. https://orcid.org/0000-0003-3668-7054
Anelise Bergmann Araujo Hospital de Clínicas de Porto Alegre – Porto Alegre (RS), Brasil. https://orcid.org/0000-0001-5277-1964
Alessandra Aparecida Paz Hospital de Clínicas de Porto Alegre – Porto Alegre (RS), Brasil. https://orcid.org/0000-0001-6429-3998
Lucia Silla Hospital de Clínicas de Porto Alegre – Porto Alegre (RS), Brasil. https://orcid.org/0000-0002-3892-6525

Resumen

Objetivo: Evaluar la seguridad y la eficacia del tratamiento tópico de heridas con células estromales mesenquimales derivadas de médula ósea cultivadas y suspendidas en lisado plaquetario. Método: Estudio prospectivo, intervencionista y controlado con 42 ratones C57BL/6 con una herida por escisión inducida. Fueron divididos en cuatro grupos de tratamiento: hidrogel (tratamiento estándar para heridas), gel salino (control), gel de lisado plaquetario y gel de lisado plaquetario que contenía células estromales mesenquimales. Los análisis se realizaron el día 7 (n=5) y el día 13 (n=7) para inmunohistoquímica, y el día 21 (n=30) para seguridad. El seguimiento se llevó a cabo mediante evaluación clínica (eventos adversos, peso, dolor, comportamiento y reducción del tamaño de la herida) e histológica (cicatrización, vascularización y colagenización). Resultados: En relación con la seguridad, no se identificaron eventos adversos ni alteraciones fisiológicas relacionadas con el tratamiento. El grupo tratado con células presentó mejores resultados de cicatrización en las evaluaciones macro y microscópicas, seguido por el grupo tratado con gel de lisado plaquetario. Conclusión: La aplicación tópica de células estromales mesenquimales fue segura y mostró resultados favorables.

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Citas

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